Purification of lactoperoxidase from creek-water buffalo milk and investigation of kinetic and antibacterial properties

Ozdemir H., HACIBEYOGLU H., USLU H.

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, cilt.32, sa.2, ss.143-155, 2002 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 2
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1081/pb-120004127
  • Dergi Adı: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.143-155
  • Atatürk Üniversitesi Adresli: Evet

Özet

Water buffalo lactoperoxidase (WBLP) was purified with Amberlite CG 50 H+ resin, CM Sephadex C-50 ion-exchange chromatography, and Sephadex G-100 gel filtration chromatography from skim milk. All purification steps of the WBLP were shown with SDS-PAGE and R-z (A(412)/A(280)) controlled the purification degree of the enzyme. R-z value for the purified WBLP was 0.8.