Nepeta meyeri essential oil ameliorates fungal infection and the antioxidant response in grapevines (Vitis vinifera) infections by gray mold (Botrytis cinerea)

Karakuş S., Atıcı Ö., Köse C., Aydin I.

ACTA PHYSIOLOGIAE PLANTARUM, cilt.43, sa.12, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 12
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s11738-021-03320-3
  • Dergi Adı: ACTA PHYSIOLOGIAE PLANTARUM
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: Antioxidant enzyme, Essential oil, Nepeta meyeri, Botrytis cinerea, Vitis vinifera, HYDROGEN-PEROXIDE, O-HYDROXYETHYLORUTIN, ANTIFUNGAL ACTIVITY, ERYSIPHE-NECATOR, REACTIVE OXYGEN, ARGOLICA BORY, PLANT-GROWTH, TOMATO, BENTH., ACID
  • Atatürk Üniversitesi Adresli: Evet

Özet

The essential oil (EO) obtained from Nepeta meyeri Benth. used to struggle allelopathically with gray mold (Botrytis cinerea Pers.), which causes significant damage to vineyards (Vitis vinifera L. cv. Karaerik). The EO from N. meyeri was determined to contain 4a alpha, 7 alpha, 7a alpha-nepetalactone (71.68%) at the highest rate when analyzed with GC/MS. Then, the doses (0.6, 0.8, and 1.0 mu L/leaf) of 1 ppm EO solution were applied to the leaves together with the fungus inoculation (EO + F), as well as 1 day (EO + F1D) and 2 days (EO + F2D) before inoculation. The fungus application alone was evaluated as a control. Seven days after the inoculations, fungus development, lipid peroxidation level (as malondialdehyde), the contents of reactive oxygen species (ROS), photosynthetic pigments and soluble proteins, and the activities of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, and glutathione reductase) were determined in the leaves. Findings revealed that EO + F1D among the applications and 1.0 mu L/leaf among the EO doses significantly prevented fungus development on the leaf, compared to the control. Moreover, the same applications decreased ROS levels, such as H2O2 and O-2(center dot-) and lipid peroxidation, while they increased antioxidant enzyme activities, as well as chlorophyll and soluble protein contents. Therefore, it can be suggested that the EO from N. meyeri has a potentially powerful usage as biofungicide in the allelopathic struggle against the infection of B. cinerea in vineyards.