Sulfanilamide Modified Magnetic Nanoparticles for Purification of Carbonic Anhydrase from Bovine Blood

Celik S., Solak K., Mavi A.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, cilt.195, sa.6, ss.3900-3913, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 195 Sayı: 6
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s12010-022-03983-9
  • Dergi Adı: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.3900-3913
  • Anahtar Kelimeler: Purification, Magnetic nanoparticles, Carbonic anhydrase, AFFINITY-CHROMATOGRAPHY, TAGGED PROTEIN, SEPARATION, NANOCOMPOSITES, EXTRACTION, DELIVERY, CAPTURE, RELEASE, BINDING, GEL
  • Atatürk Üniversitesi Adresli: Evet

Özet

Magnetic nanoparticles (MNPs) have been used for purification of specific biomolecules form mixtures. The aim of this study is to develop a new, cheap, reusable, and magnetic-based material to purify the carbonic anhydrase (CA) enzyme in a short time with high efficiency. In the first part of this study, silica-coated iron oxide magnetic nanoparticles (Fe3O4@SiO2 MNPs) were obtained. Surface modification of Fe3O4@SiO2 MNPs was accomplished with 3-(4-Hydroxyphenyl) propionic acid (PA) and sulfanilamide (SA), respectively. SA is a selective inhibitor of CA, and it selectively binds to CA. The final particle was named Fe3O4@SiO2-PA-SA MNPs and characterized by SEM, TEM, XRD, and FT-IR. It was determined that the produced MNPs contained multicore, were smaller than 100 nm in size, and had a spherical morphology. The CA was purified from bovine blood hemolysate in a short time such as 2.5 h and in a simple manner. The maximum enzyme purifying capacity of MNPs was calculated as 13.87 +/- 3.27 mg CA/g MNP. SDS-PAGE analysis was confirmed that high CA purification success was achieved.