Akademik Veri Yönetim Sistemi
World Congress on Applied Nanotechnology, Erzurum, Türkiye, 24 - 26 Kasım 2021
Researchers have recently focused on using microbes as "nanofactories" for the
manufacture of metal nanoparticles. Bacterial synthesis of nanoparticles (NPs) is a green
chemistry approach that interconnects nanotechnology and microbial biotechnology. The
use of bacteria for production nanoparticles provides a low cost, simple and eco-friendly
method because the synthesis occurs under ambient conditions without the use of toxic
reducing agents. The resistance of pathogenic bacteria to antibiotics has become a serious
problem. Therefore, the development of novel and potent bactericidal agents is of great
importance. In the present study, zinc selenide nanoparticles (Zn:Se-NPs) have been
synthesized by Pseudomonas aeruginosa strain OG1 in Nutrient Broth (NB) medium
containing sodium selenite and zinc sulfate under dark conditions. The antibacterial activities
of nanoparticles at different concentrations (50-250 μg/mL) against both Gram-negative
(Escherichia coli and Acinetobacter baumannii) and Gram-positive bacteria (Bacillus cereus
and Staphylococcus aureus) were tested in NB medium. After 24 h treatment, nanoparticles
inhibited the growth of both bacterial groups in a concentration-dependent manner. The
growth curves of bacterial cells treated with nanoparticles indicated that nanoparticles could
inhibit the reproduction of bacterial cells. This study indicates that Zn:Se-NPs can be used as
effective antibacterial agents against various microorganisms which can endanger human
beings.