Identification of Thermophilic Strains from Geothermal Areas in Turkey by using Conventional and Molecular Techniques

YANMIS D., BALTACI M. Ö., GÜLLÜCE M., ADIGÜZEL A.

RESEARCH JOURNAL OF BIOTECHNOLOGY, cilt.10, sa.1, ss.39-45, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 10 Sayı: 1
  • Basım Tarihi: 2015
  • Dergi Adı: RESEARCH JOURNAL OF BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.39-45
  • Anahtar Kelimeler: Thermophilic bacilli, characterization, FAMEs, 16S rRNA gene, BOX-PCR, ESCHERICHIA-COLI, RETAIL BEEF, REP-PCR, PURIFICATION, BACTERIA, ERZURUM, DIFFERENTIATION, BACILLI
  • Atatürk Üniversitesi Adresli: Evet

Özet

A survey of isolation was practised by obtaining water samples from various thermal facilities in order to find out new types of thermophilic bacteria and to characterize them via phenotypic and genotypic methods. Hence, the test strains were first grouped according to their colonial morphologies and were characterized via conventional (morphological, physiological, biochemical) and molecular [Fatty Acid Methyl Ester profiles (FAME), 16S rRNA sequencing and BOX-PCR] methods. As the result of the research, it was determined that FAMEs method failed in the identification of thermophilic bacteria at the species level whereas BOX-PCR technique was quite successful in exhibiting genetic differences among thermal isolates.