Akademik Veri Yönetim Sistemi
ROMANIAN BIOTECHNOLOGICAL LETTERS, cilt.22, sa.4, ss.12741-12750, 2017 (SCI-Expanded)
The present study was carried out in order to elucidate whether exogenous ALA treatment mitigates the stress damages in wheat under boron toxicity. Three different concentrations (5, 10 and 20 mg.L-1) of ALA were applied to the 9-day-wheat leaves and then boron (5 mM) was added to the nutrient solutions for 3 days. Boron toxicity severely reduced root and shoot lengths as well as the level of soluble protein and photosynthetic pigments. In a similar manner, it caused a markable decrease in the activities of antioxidant enzymes including superoxide dismutase, guaicol peroxidase, catalase, ascorbate peroxidase and glutathione reductase. As a result, boron toxicity led to increment in the level of oxidative stress indicators such as superoxide anion, hydrogen peroxide, and lipid peroxidation. ALA application significantly mitigated the boron-induced reductions in the growth parameters and antioxidant defence system. The values of oxidative stress indicators were lesser in ALA-applied seedlings that were associated with higher activities of antioxidant enzymes as well as higher protein level. A significant correlation was recorded between boron and/or ALA-induced protein content and protein profile observed at SDS-PAGE. On the other hand, while boron application caused to a marked increase in boron amounts in roots, ALA treatments reduced considerably the boron uptake from roots. These data clearly revealed that ALA improves boron toxicity in wheat seedlings by increasing antioxidant activity and by modulating boron uptake and/or transportation.