Analysis of fluorescence quenching of pyronin B and pyronin Y by molecular oxygen in aqueous solution

Celebi N., Arik M., Onganer Y.

JOURNAL OF LUMINESCENCE, cilt.126, sa.1, ss.103-108, 2007 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 126 Sayı: 1
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.jlumin.2006.05.009
  • Dergi Adı: JOURNAL OF LUMINESCENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.103-108
  • Anahtar Kelimeler: pyronin B and pyronin Y, dynamic and static quenching, molecular oxygen
  • Atatürk Üniversitesi Adresli: Evet

Özet

The fluorescence quenching of pyronin B and pyronin Y molecules by molecular oxygen in aqueous solution was studied by using steady-state and time-resolved fluorescence and UV-Vis absorption spectroscopy techniques. In order to understand the quenching mechanism, fluorescence decays, absorption and fluorescence spectra of the probes were recorded as a function of the oxygen concentration and temperature. The quenching was found to be appreciable and shows positive deviation in the Stern-Volmer representation obtained from the fluorescence intensity ratio. Fluorescence quenching constants (k(q)) were calculated from the tau(o)/tau vs. [Q] plots having linear correlation and compared with calculated diffusion-controlled rate constants (k(diff)) values. Experimental results were in good agreement with the simultaneous dynamic and static quenching model. (C) 2006 Elsevier B.V. All rights reserved.