Production of a Novel Pectin Lyase From Bacillus pumilus (P9), Purification and Characterisation and Fruit Juice Application

NADAROĞLU H., Taskin E., ADIGÜZEL A., GÜLLÜCE M., DEMIR N.

ROMANIAN BIOTECHNOLOGICAL LETTERS, cilt.15, sa.2, ss.5167-5176, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 2
  • Basım Tarihi: 2010
  • Dergi Adı: ROMANIAN BIOTECHNOLOGICAL LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.5167-5176
  • Anahtar Kelimeler: Pectin lyase, Purification, Bacillus pumilus (P9), Pectin, Juice Clarification, SOLID-STATE, INDUSTRIAL APPLICATIONS, FUNGAL STRAINS, ENZYMES, POLYGALACTURONASE, FERMENTATION, OPTIMIZATION, PRETREATMENT, PROTEINS, BACTERIA
  • Atatürk Üniversitesi Adresli: Evet

Özet

Extracellular pectin lyase (EC 4.2.2.10) was produced by Bacillus pumilus (P9) in solid state fermentation. Pectin lyase enzyme was purified 3636 fold by using DEAE-cellulose anion exchange column chromatography and characterized. Molecular weight of the enzyme was determined as 25 kDa by using Sephadex G-100 gel filtration chromatography.