Waste frying oil hydrolysis and lipase production by cold-adaptedPseudomonas yamanorumLP2 under non-sterile culture conditions

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Komesli S., Akbulut S., Arslan N. P., Adıgüzel A., Taşkın M.

ENVIRONMENTAL TECHNOLOGY, cilt.42, sa.20, ss.3245-3253, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 42 Sayı: 20
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1080/09593330.2020.1745297
  • Dergi Adı: ENVIRONMENTAL TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, EMBASE, Environment Index, Geobase, Greenfile, INSPEC, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, DIALNET, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.3245-3253
  • Anahtar Kelimeler: Pseudomonas yamanorumLP2, cold-adapted, lipase, non-sterile culture, waste frying oil, HEAVY-METAL BIOSORPTION, MOLECULAR CHARACTERIZATION, INDUSTRIAL APPLICATIONS, MICROBIAL LIPASES, RHIZOPUS-ORYZAE, CARBON-SOURCES, LACTIC-ACID, OPTIMIZATION, PSYCHROTOLERANT, FERMENTATION
  • Atatürk Üniversitesi Adresli: Evet

Özet

Non-sterile culture technique is currently used in some microbial processes. However, there is no study on the use of this technique in the production of microbial lipases and hydrolysis of waste frying oils. This study was conducted to hydrolyse waste frying oils and produce lipase under non-sterile culture conditions using locally isolated cold-adapted bacteria. Of 75 bacterial isolates, the psychrotolerantPseudomonas yamanorumLP2 (Genbank number: KU711080) was determined to have the highest lipase activity. It was found that a combination of restricted nutrient availability, low temperature and high inoculum volume prevented microbial contaminants under non-sterile conditions. The most favourable parameters for lipase production under both sterile and non-sterile conditions were 15 degrees C temperature, pH 8, 30 mL/L inoculum volume, 40 mL/L waste frying oil concentration, 10 mL/L Tween-80 and 72 h incubation time. The maximum lipase activities in sterile and non-sterile media were determined as 93.3 and 96.8 U/L, respectively. The present process designed for enzyme production and waste oil hydrolysis can reduce the cost of cultivation medium as well as energy consumption and workload. The potential of cold-adapted bacteria to produce lipase and hydrolyse waste oils under non-sterile culture conditions was first tested in the current study.