Therapeutic effect of Berberis vulgaris fruit extract on histopathological changes and oxidative stress markers of ovarian ischemia and reperfusion injury in rats
Journal of King Saud University - Science, cilt.36, sa.11, 2024 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 36 Sayı: 11
- Basım Tarihi: 2024
- Doi Numarası: 10.1016/j.jksus.2024.103578
- Dergi Adı: Journal of King Saud University - Science
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, zbMATH, Directory of Open Access Journals
- Anahtar Kelimeler: Berberis vulgaris, Ischemia, Ovarian, Reperfusion, TNF-α
- Atatürk Üniversitesi Adresli: Evet
Özet
Aim: This study aimed to investigate the possible protective effect of berberine, the active compound of Berberis vulgaris plant extract, which has anti-inflammatory and antioxidant properties, in an ovarian ischemia–reperfusion model by utilizing molecular, biochemical, and histopathological methods. Methods: In this experimental study, 42 adolescent female Sprague Dawley rats (6 weeks old) were divided into 7 equal groups. Under anesthesia, ischemia was induced by ligating the bilateral adnexal tissues, and after 3 h of ischemia, reperfusion was achieved by removing the adnexal ligation. Berberis vulgaris extract was administered by oral gavage 1 h after ischemia for the ischemia groups and 1 h before reperfusion for the ischemia + reperfusion groups. After 3 h of reperfusion, the experiment was terminated. Results: The administration of Berberis vulgaris extract decreased the hemorrhagic areas, reduced the number of TUNEL-positive cells, and decreased CYC1 immunoreactivity levels in histopathological analysis compared to the groups subjected to ischemia or ischemia + reperfusion without the plant extract. CAT, SOD, and GSH increased due to Berberis vulgaris administration while MDA levels decreased. Berberis vulgaris also downregulated TNF-α levels compared to the ischemia and ischemia + reperfusion groups. Conclusion: Berberis vulgaris extract may have a protective effect against ovarian ischemia–reperfusion injury. Further molecular studies are needed to clarify this protective effect.