Could Vital Dyes be used to Determine the Degree of the Time Dependent Viability Changes in Cryptosporidium parvum Oocysts?

YILMAZER N., GÜVEN E., Kucuk S. K., Dittmar K., Bangoura B., KAR S.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.17, sa.6, ss.953-956, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 6
  • Basım Tarihi: 2011
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.953-956
  • Atatürk Üniversitesi Adresli: Evet

Özet

The present study was undertaken to determine time dependent viability changes of purified Cryptosporidium parvum oocysts, stored in antimicrobial-supplemented PBS at +4 degrees C, using vital dyes (DAPI/PI). The trials demonstrated that vital dyes could provide estimation of oocyst viability, and furthermore, if interpreted correctly, they could be used to determine the degree of the viability in Cryptosporidium parvum oocysts as cell culture-PCR assay is used.