Akademik Veri Yönetim Sistemi
4 TH INTERNATIONAL CONGRESS ON ENGINEERING AND LIFE SCIENCE “Life in the Grip of Pandemic and War: Agriculture and Nature”, Comrat, Moldova, 17 - 19 Kasım 2023, ss.241-247
Abstract: Acrylamide (ACR) is a chemical that can pose a serious carcinogenic risk and is formed during thermal
processes, such as the high-temperature cooking or frying of starchy foods. Exposure to products created through these
means causes damage to individuals. ACR exposure cause to damage in various tissues and organs. Additionally, the
observed gonadotoxic effects in experimental animals emphasize the potential harms of this substance. Melatonin (MEL)
is a versatile compound secreted by the pineal gland in vertebrates, with various effects and functions. It has been found
to play a significant role in regulating steroid hormone secretion in the male reproductive process. Therefore, this study
investigated the effect of MEL on ACR-induced testicular toxicity in rats. Rats were randomly divided into five groups:
The Control, ACR, MEL10+ACR, MEL20+ACR, and MEL20 group. In the ACR groups, ACR was administered at a
dose of 50 mg/kg, while in the MEL groups, MEL was administered at doses of 10 mg/kg and 20 mg/kg. The experiment
continued for 14 days, and it was concluded on the 15th day. At the end of the experiment, testicular tissues obtained
from the rats were placed in -80°C freezer. Testicular tissues removed from the freezer on the day of the experiment were
subjected to homogenization. The resulting supernatants were analyzed according to the ELISA protocol. It was observed
that exposure to ACR increased COX-2, iNOS, and PGE2 levels in the supernatants prepared from the tissues of rats
exposed to ACR. ACR exposure was found to cause testicular tissue damage in rats. The application of MEL along with
ACR significantly reduced the testicular toxicity induced by ACR. Melatonin exhibited anti-inflammatory effects by
reducing COX-2, iNOS, and PGE2 levels. However, we believe that conducting more detailed research at this level would
be appropriate.
Keywords: Acrylamide, Gonadotoxic, Inflammation, Testis, Rat