Akademik Veri Yönetim Sistemi
FRESENIUS ENVIRONMENTAL BULLETIN, cilt.27, sa.11, ss.7222-7229, 2018 (SCI-Expanded)
This study was prepared to determine reactions of mammalian sexual hormones (MSH) (progesterone, 17 beta-estradiol, estrone and testosterone) on sain-foin plants (Onobrychis sativa L.) grown in in vitro conditions, which was applied for 13 different methods performed in two stages. In the first stage, the seeds were cultured in hormone-free medium. In the second stage, the explants (cotyledon, root and hypocotyl) obtained from the plantlets were transferred to the medium including different hormone, dose and hormone-dose combinations. For the effects of MSH, the highest shoot (16 shootlpetri) were obtained from hypocotyl explants with the doses 10(-6) mM of progesterone and, 10(-4) and 10(-6,)mM of estrone; the highest rate of callus formation (84,4 %) with the dose 10(-4) mM of estrone; the highest vigor rate (73,4%) with the doses 10(-4) and 10(-5) mM of progesterone from root explants were obtained. For rooting, the most suitable hormone was testosterone, the most suitable explant was hypocotyl and it is observed that number of root increased depending on treatment day. This study is the primary in terms of determination of the effects of MSH on tissue culture of sainfoin (Onobrychis sativa L).