Characterization of Water-Soluble Extracellular Polysaccharide from <i>Aeribacillus pallidus</i> IM17

Genc B., TUNÇ M. T., ADIGÜZEL A.

INDIAN JOURNAL OF MICROBIOLOGY, cilt.64, sa.3, ss.973-982, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 64 Sayı: 3
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1007/s12088-023-01178-2
  • Dergi Adı: INDIAN JOURNAL OF MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Veterinary Science Database
  • Sayfa Sayıları: ss.973-982
  • Anahtar Kelimeler: Aeribacillus pallidus, Emulsification, Foaming, Rheology, X-ray diffraction
  • Atatürk Üniversitesi Adresli: Evet

Özet

The production of the extracellular polysaccharide from the thermophilic bacterium Aeribacillus pallidus was carried out in the study. The polysaccharide was isolated and characterized by means of GC-MS, FT-IR, DSC, and XRD analyses. The rheological, foaming, and emulsifying properties of the polysaccharide were determined. Using a sucrose-rich medium, 27.1 mg dried EPS/100 mL was obtained with 94% carbohydrate and 1.5% protein. The monosaccharide profile of water-soluble EPS-IM17 was composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose. The foaming capacity and stability of EPS-IM17 were 26.67% (+/- 4.71) and 40.01% (+/- 4.95), respectively, and the foaming stability was not affected by time. The emulsification index of EPS-IM17 was 64.54 (+/- 8.71) and decreased to 38.47 (+/- 10.44) after 24 h. EPS-IM17 had a crystalline structure. Solutions at different concentrations (10, 20, 40 mgmL(-1)) showed pseudoplastic behavior. In conclusion, this report could be a lead study for the use of Aeribacillus pallidus extracellular polysaccharide for different applications.