Akademik Veri Yönetim Sistemi
SYMBIOSIS, cilt.38, sa.2, ss.163-174, 2005 (SCI-Expanded)
We investigated effect of putrescine on inducing symbiosis of strains of Rhizobium leguminosarum in commercial inoculants and strains isolated from local soils inoculated to roots of chickpea (Cicer arietinum cv. Aziziye-94) or vetch (Vicia sativa cv. Karaelci). In the growth medium of the plants inoculated with the strains of R. leguminosarum, putrescine (10 muM) was applied on the 10th day after planting. Growth parameters and symbiotic characteristics were determined 45 days after the inoculations. Nodule number, dry weight of nodules, roots and shoots, and nitrogen and chlorophyll contents significantly varied between putrescine-treated and control plants. The putrescine treatment increased the dry weight accumulation of nodules and shoots of both plants studied by more than 40%. Total nitrogen and chlorophyll contents also were significantly enhanced in response to putrescine treatment. Further, the stimulatory effect of putrescine was not strain related (commercial inoculants and strains isolated from local soils). In conclusion, the trials under controlled environmental conditions showed that the 10 muM putrescine application in plant growth medium significantly caused an increase in symbiotic parameters of chickpea and vetch inoculated with both commercial inoculants and indigenous strains of R. leguminosarum.