Autofluorescence-Based Investigation of Spatial Distribution of Phenolic Compounds in Soybeans Using Confocal Laser Microscopy and a High-Resolution Mass Spectrometric Approach

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Razgonova M. P., Zinchenko Y. N., Kozak D. K., Kuznetsova V. A., Zakharenko A. M., ERCİŞLİ S., ...Daha Fazla

Molecules, cilt.27, sa.23, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 23
  • Basım Tarihi: 2022
  • Doi Numarası: 10.3390/molecules27238228
  • Dergi Adı: Molecules
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Directory of Open Access Journals, Civil Engineering Abstracts
  • Anahtar Kelimeler: Glycine Willd, flavonols, laser microscopy, HPLC-MS, MS, tandem mass spectrometry, polyphenols, COWPEA VIGNA-UNGUICULATA, HPLC-DAD, ANTIOXIDANT ACTIVITY, SECONDARY METABOLITES, CELL-WALLS, SEED COATS, IDENTIFICATION, L., QUANTIFICATION, POLYPHENOLS
  • Atatürk Üniversitesi Adresli: Evet

Özet

© 2022 by the authors.In this research, we present a detailed comparative analysis of the bioactive substances of soybean varieties k-11538 (Russia), k-11559 (Russia), k-569 (China), k-5367 (China), k-5373 (China), k-5586 (Sweden), and Primorskaya-86 (Russia) using an LSM 800 confocal laser microscope and an amaZon ion trap SL mass spectrometer. Laser microscopy made it possible to clarify in detail the spatial arrangement of the polyphenolic content of soybeans. Our results revealed that the phenolics of soybean are spatially located mainly in the seed coat and the outer layer of the cotyledon. High-performance liquid chromatography (HPLC) was used in combination with an amaZon SL BRUKER DALTONIKS ion trap (tandem mass spectrometry) to identify target analytes in soybean extracts. The results of initial studies revealed the presence of 63 compounds, and 45 of the target analytes were identified as polyphenolic compounds.