The toxicological effects of some avermectins on goat liver carbonic anhydrase enzyme

Caglayan C., GÜLÇİN İ.

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, cilt.32, sa.1, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 1
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1002/jbt.22010
  • Dergi Adı: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: avermectin, carbonic anhydrase, enzyme inhibition, enzyme purification, goat liver, ERYTHROCYTES IN-VITRO, INHIBITORY PROPERTIES, ISOENZYMES I, LIQUID-CHROMATOGRAPHY, ACETYLCHOLINESTERASE, ANTIOXIDANT, DERIVATIVES, MOXIDECTIN, IVERMECTIN, DORAMECTIN
  • Atatürk Üniversitesi Adresli: Evet

Özet

Avermectins are used worldwide as antiparasitic drugs in the field of veterinary medicine and as agricultural pesticides and insecticides. Carbonic anhydrase (CA, E.C. 4.2.1.1) is a zinc-containing metalloenzyme that catalyzes the reversible hydration of carbon dioxide (CO2) to yield protons (H+) and bicarbonate (HCO3-). In this study, some avermectins, including abamectin, doramectin, eprinomectin, and moxidectin, were investigated for in vitro inhibitory effects on the CA enzyme purified from goat liver, which was purified (125.00-fold) using sepharose 4B-l-tyrosine-sulfanilamide affinity chromatography, with a yield of 68.27% and a specific activity of 21765.31 EU/mg proteins. The inhibition results obtained from this study showed K-i values of 0.283, 0.153, 0.232, and 0.317 nM for abamectin, doramectin, eprinomectin, and moxidectin, respectively. On the other hand, acetazolamide, well-known clinically established CA inhibitor, possessed a K-i value of 0.707 nM against goat liver CA.