Akademik Veri Yönetim Sistemi
Dros. Inf. Serv., cilt.103, sa.1, ss.1-8, 2020 (Hakemli Dergi)
Lactarius indigo is an edible fungus and contains various secondary metabolites. One of these metabolites, Guaiazulene (GUA), is a kind of terpene. In this study, it was observed that Drosophila melanogaster shortened the life span of both male and female populations based on Guaiazulene's dose-time interaction (25, 50, 100, 200 ppm GUA and chronic application every three days). The average life span in the control group was 48.83 ±1.95 days in the ♀ population and 48.30±2.22 days in the ♂ population. These values in the ♀♀ were found to be 44.91±1.45 to 12.11±0.26 in the lowest and highest Gua application groups(25-200 ppm); in ♂♂, it decreased from 44.70±1.22 to 11.44±0.21 (P<0.05). In the second part of the study, water extracts of two edible fungi, Pleurotus sajor-caju (PSCwt) and Pleurotus osteratus (POwt), were used together with GUA (200 ppm GUA+PSCwt and GUA+POwt, 1:1 v/v ratio). Both extracts showed a healing effect and increased longevity compared to 200 ppm GUA application. Namely, in GUA+PSCwt application, the longevity was extended to 21.93±0.62 days for females and 19.05±0.46 days for males.These values were found as 21.83±0.66 and 18.75±0.39 days in GUA+POwt application, respectively (P<0.05). In this study, both fungal extracts were also analyzed by gas chromatography-mass spectrometry (GS-MS). A total of 13 and 10 components were identified in POwt and PSCwt, respectively.In addition, total phenol and flavanoid contents of two extracts were determined.