Genotyping of<i> Brucella</i> isolates from animals and humans by Multiple-Locus Variable-number Tandem Repeat Analysis (MLVA)

Ozmen M., Ozgen E. K., Sayi O., Putur E. K., Okumus B., Yilmaz S. I., ...Daha Fazla

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, cilt.96, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 96
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.cimid.2023.101981
  • Dergi Adı: COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, Environment Index, Veterinary Science Database
  • Anahtar Kelimeler: B, melitensis, MLVA-16, Ruminant, Turkiye, MOLECULAR-IDENTIFICATION, BIOVAR 3, MELITENSIS, ABORTUS, BACTEREMIA, INFECTION, BOVINE, OVINE
  • Atatürk Üniversitesi Adresli: Evet

Özet

This study investigates country-wide genotype variations through the genotyping of Brucella strains isolated from domestic ruminants and humans. The Brucella spp. isolated from samples taken from animals and humans were first identified as B. abortus and B. melitensis by real-time PCR, and the MLVA-16 approach was then used for the genotyping of the identified isolates. For the study, 416 Brucella spp. were isolated from aborted fetus samples examined between 2018 and 2021, and 74 Brucella spp. from infected humans. Of the 74 human isolates analyzed, 1.3% were identified as B. abortus and 98.7% (73/74) as B. melitensis. The MLVA-16 typing method revealed 30 clonal groups for B. abortus and 37 clonal groups for B. melitensis from which the dominant genotypes and similarities with human isolates in Turkiye were determined.