Preparation of Chitosan with High Antibacterial Efficiency from Penicillium crustosum TZ18

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Kayar F., Doymuş M., Özkan H., Arslan N. P., Adıgüzel A., Taşkın M.

JOURNAL OF POLYMERS AND THE ENVIRONMENT, cilt.30, sa.9, ss.3598-3608, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 9
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s10924-022-02458-6
  • Dergi Adı: JOURNAL OF POLYMERS AND THE ENVIRONMENT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aerospace Database, BIOSIS, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, Environment Index, Geobase, Greenfile, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.3598-3608
  • Anahtar Kelimeler: Fungi, Chitosan, Production, Characterization, CITRIC-ACID PRODUCTION, SUGAR-BEET MOLASSES, MOLECULAR-WEIGHT, RHIZOPUS-ORYZAE, FUNGAL CHITOSAN, LACTIC ACID, CHITIN, EXTRACTION, DEACETYLATION, HYDROLYSATE
  • Atatürk Üniversitesi Adresli: Evet

Özet

Fungi are accepted as novel sources of chitinous materials. The present study was performed to produce chitinous material from locally isolated Penicillium crustosum TZ18 (GenBank accesion number: OM095448) using molasses as substrate, prepare chitosan from chitinous material and investigate the antibacterial activity of the prepared chitosan. An initial pH of 6.0, a molasses concentration of 120 g/L, a temperature of 25 degrees C and an incubation time of 5 days were the most favourable parameters for the production of chitinous material. Under the optimized culture conditions, fungal biomass of 29.5 g/L and total chitinous material of 7.79 g/L (26.4%) could be produced. The chitin and chitosan concents of chitinous material were 97 (7.56 g/L) and 3% (0.23 g/L), respectively. Chitin fraction was converted into the chitosan (Penicillium chitosan, PC) by deacetylation process. FT-IR results revealed that the functional groups of PC and commercial chitosan (CC) were very similar to each other. Deacetylation degrees of PC and CC were found to be 78.3 and 76.9%, respectively. The viscosity-average molecular weights of PC and CC were determined as 122.7 and 298.3 kDa, respectively. When compared to CC, PC was found to have higher antibacterial efficiency against both Escherichia coli and Staphylococcus aureus. High antibacterial efficiency of the chitosan could be ascribed to its low molecular weight and high deacetylation degree. The chitosan may be useful for biomadical applications. The production of chitinous material from P. crustosum and the antibacterial efficiency of P. crustosum chitosan were investigated for the first time in the present study.