Akademik Veri Yönetim Sistemi
BIOSCIENCE RESEARCH, cilt.17, sa.2, ss.775-785, 2020 (ESCI)
In this work, the development of zinc biosensors based on zinc nanoparticles (Zn NPs) biomimetic enzymes were carried out to spectrophotometrically detect glucose in food samples. Briefly; Glucose oxidase enzyme reacts with glucose in food samples to form gluconic acid and H2O2. Behind; the peroxide formed can be measured spectrophotometrically using ABTS substrate with the enzyme mimic effect of Zn NPs. In this way, the amount of glucose was determined by a simple, effective and selective method by measuring the change in absorbance of the reaction medium, which changed by oxidation. ZnO NPs were obtained from Zn(NO3)(2) by green synthesis method using raw fig extract. Then, the amount of glucose in some food samples was determined using ZnO NPs. The results showed that the glucose content in the samples was exactly and reproducibly measured with a correlation coefficient of 0.9812 at 3.47 mM-27.78 mM concentrations. This will greatly simplify the design and manufacture of the new biosensor, which can be used for glucose fixation in food samples in an inexpensive, efficient, environmentally friendly and fast manner.