Genetic diversity in grapevine germplasm resources in the Coruh valley revealed by RAPD markers

Ercişli S., Orhan E., Hızarcı Y., Yildirim N., Ağar G.

BIOCHEMICAL GENETICS, cilt.46, ss.590-597, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 46
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1007/s10528-008-9173-7
  • Dergi Adı: BIOCHEMICAL GENETICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.590-597
  • Anahtar Kelimeler: Vitis vinifera, RAPD (random amplified polymorphic DNA), genetic diversity, AMPLIFIED POLYMORPHIC DNA, MANGO CULTIVARS, IDENTIFICATION
  • Atatürk Üniversitesi Adresli: Evet

Özet

Random amplified polymorphic DNA analysis was carried out in 35 autochthonous table grapevine cultivars grown in Coruh valley. Fifty-five oligonucleotide primers were screened on cultivars, and among them, 12 primers showed clear polymorphic patterns. PCR amplification with 12 primers generated a total of 157 polymorphic bands. There was genetic variation among the cultivars with values of genetic diversity ranging from 0.19 to 0.72 using the Jaccard coefficient. UPGMA analysis of the distance matrix resulted in a dendrogram with two main clusters. The first cluster included 28 cultivars and the second 7 cultivars. The greatest genetic similarity was observed between cultivars Gah and Kolik, while the greatest dissimilarity was observed between cultivars Gah and Siyah Kus Uzumu. The dendrogram revealed that the cultivars present in Coruh valley can be distinguished to a relatively high degree.