Identification of apricot cultivars in Turkey (Prunus armeniaca L.) using RAPD markers

ERCİŞLİ S., AĞAR G., YILDIRIM N., EŞİTKEN A., ORHAN E.

ROMANIAN BIOTECHNOLOGICAL LETTERS, cilt.14, sa.4, ss.4582-4588, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 4
  • Basım Tarihi: 2009
  • Dergi Adı: ROMANIAN BIOTECHNOLOGICAL LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4582-4588
  • Anahtar Kelimeler: Prunus armeniaca L, Cluster analysis, Genetic diversity, RAPD, MOLECULAR CHARACTERIZATION, GENETIC-RELATIONSHIPS, VARIETIES, RESOURCES, GERMPLASM, DIVERSITY
  • Atatürk Üniversitesi Adresli: Evet

Özet

This study was carried out to determine genetic relationships among 23 apricot cultivars from Turkey by using randomly amplified polymorphic DNA (RAPD) technology. Among the 40 decamer random primers were used for PCR reactions, 12 primers showed reproducible polymorphic patterns. These primers produced 121 bands, out of which 118 were polymorphic. UPGMA (Unweighted Pair Group Method using Arithmetic average) derived dendrogram, grouped the cultivars into one cluster and one outlier. The main cluster further divided into three subclusters. The highest dissimilarities found between Hacihaliloglu and Kabaasi cultivars (0.108). The variation detected among the closely related genotypes indicates the efficiency of RAPD markers over the morphological and isozyme markers for the identification and construction of genetic linkage maps.