Purification and characterization of an extracellular cellulase from Anoxybacillus gonensis O9 isolated from geothermal area in Turkey
JOURNAL OF ENVIRONMENTAL BIOLOGY, cilt.36, sa.6, ss.1319-1324, 2015 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 36 Sayı: 6
- Basım Tarihi: 2015
- Dergi Adı: JOURNAL OF ENVIRONMENTAL BIOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1319-1324
- Anahtar Kelimeler: Anoxybacillus gonensis, 16S rRNA sequencing, Cellulase, Characterization, SP NOV., BACILLUS, SEQUENCE
- Atatürk Üniversitesi Adresli: Evet
Özet
In the present study, cellulase was purified and characterized from Anoxybacillus gonensis (Gen bank Number: KM596794) which was isolated and characterized from Agri Diyadin Hot spring. It was found to synthesize cellulase which had a wide range of industrial applications. Twenty four-hour-cultured bacteria induced cellulase production and specific activities during the purification steps were 1.47, 81.06 and 109.4 EU mg(-1) protein at crude extract, ammonium sulphate precipitated and DEAE-Sephadex purification steps. The highest enzyme activity was observed at 50 degrees C and the optimum range of pH was 3-10. Molecular weight of enzyme was determined approximately 40kDa. The kinetic parameters of cellulase against carboxymethylcellulose (CMC) were 153.4 mu mol min(-1) mg for V-max and 0.46mM for K-m. Among effectors of the enzyme, Zn2+, Ca2+, Co2+ and EDTA decreased enzyme activity.