Impacts of high-dose riboflavin on cytotoxicity, antioxidant, growth, reproductive gene expressions, and genotoxicity in the rainbow trout gonadal cells

Çiçek S., Işık S.

TOXICOLOGY IN VITRO, cilt.94, ss.1-9, 2024 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 94
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1016/j.tiv.2023.105730
  • Dergi Adı: TOXICOLOGY IN VITRO
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Environment Index, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1-9
  • Atatürk Üniversitesi Adresli: Evet

Özet

Riboflavin (vitamin B2 found in food) is a precursor of flavin mononucleotide (FMN) and flavin adenine dinu-
cleotide (FAD), which study as coenzymes for a variety of cellular processes including biosynthesis, homocys-
teine metabolism, detoxification, and various oxidation and reduction reactions. Although studies on the
symptoms resulting from riboflavin deficiency are intense, studies on the effects of high doses of riboflavin are
almost absent. This report aimed to examine the actions of riboflavin on cell viability, the transcriptional ex-
pressions of antioxidant enzyme (gsr and gpx1a), growth (gh1, igf1, and igf2), the reproductive (bol) genes and
DNA damage in the rainbow trout gonad cells (RTG-2) for 48 h. All concentrations of riboflavin (3.125, 6.25,
12.5, 25, 50, and 100 μM) significantly reduced the RTG-2 cell viability. Riboflavin (LD50: 12.5 μM) significantly
downregulated the transcriptional expressions of gpx1a, igf1, and bol genes, while it non-significantly upregu-
lated or downregulated the transcriptional expression of gsr, igf2, and gh1 genes in the RTG-2 cells in comparison
to the control group for 48 h. The comet assay demonstrated that riboflavin significantly raised tail DNA% >10%
DMSO (positive control). Based on the outcomes, high doses of riboflavin exhibit the potential to have a role in
cellular mechanisms, including especially reproduction, DNA damage, and cell death