Hepatoprotective Effect of 17 beta-Estradiol as Antioxidant Modulators Against Stress Damage


Can S., Cıgsar G., Fatma Gür O., Aksak K., Sellı J., Bacak G., ...Daha Fazla

HEPATITIS MONTHLY, cilt.15, sa.2, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 2
  • Basım Tarihi: 2015
  • Doi Numarası: 10.5812/hepatmon.22633
  • Dergi Adı: HEPATITIS MONTHLY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Antioxidants, Exercise, Estrogens, Liver, EXERCISE-INDUCED MUSCLE, OXIDATIVE STRESS, NEUTROPHIL INFILTRATION, SKELETAL-MUSCLE, ESTROGEN, METABOLISM, ESTRADIOL, ACID, INCREASES, PERIOD
  • Atatürk Üniversitesi Adresli: Evet

Özet

Background: Liver is one of the most important organs affected by exercise. According to the literature a few study to date has investigated the effects of estrogen supplementation on exercise-induced oxidative stress in liver tissue of rats.
Objectives: We aimed to investigate the effects of estrogen supplementation on oxidative stress markers in liver tissue of exercised rats.
Materials and Methods: Male rats (n = 35) were divided as estrogen supplemented (n = 18) and non-supplemented groups (n = 17); these groups were further divided as rest and eccentric exercised groups. Eccentric exercise groups were further divided as rats killed after 1 hour and 48 hours of eccentric exercise. Estrogen (10 mg/kg) was administered subcutaneously for 30 days. Eccentric exercise was applied as treadmill run (15° downhill, 20 m/min) consisting of periods of 5 min run and 2 min rest repeated 18 times. The rat liver was examined biochemically and histologically. Activities of GST, GSH-Px, CAT, SOD and MDA concentration were also measured spectrophotometrically.
Results: Some disruptions were detected in experimental groups compared with the control group. Additionally, exercise training caused an increase in SOD and decrease in GSH-Px activities in some experimental groups. SOD activities increased significantly in group 3 (Estrogen (-), eccentric exercise (+) killed (after 1 h), compared with group 5 (Estrogen (-), eccentric exercise (+) killed (after 48 h). On the other hand, GSH-Px activities were also significantly decreased in groups 3, 4 and 5 compared with the control group. Leukocyte infiltration in liver increased after 48 hours compared with after 1 hour and estrogen supplementation was not able to prevent this infiltration.
Conclusions: Estrogen seemed to be not very effective to prevent eccentric exercise-induced liver damage.