Akademik Veri Yönetim Sistemi
1st International Erciyes Agriculture, Animal Food Sciences Conference, Kayseri, Türkiye, 24 - 27 Nisan 2019, ss.164
Colorado potato beetle,Leptinotarsa decemlineataSay (Coleoptera: Chrysomelidae) is one of the major pest that cause significant problems in potato production areas. In order to control the pest and to prevent losses, the chemical control method is the most preferred control method. However, insecticide resistance in the pest makes the chemical control difficult. In this study, imidacloprid resistance levels of Coloradopotato beetle populations collected from Afyonkarahisar province which is one of the important potato production areas in our country wasdetermined by topical application method. In all experiments, 3rd instar larvae of Coloradopotato beetle were used. Five or six doses were used for each population. Preliminary experiments were carried out to determine the doses that cause 90% mortality rate for the populations. The other doses were obtained from the first dose by serial dilutions of 50% with pure water. The concentrations were applied as 1 μl to the thorax of the larvaes. Three replications for each dose and five individuals in each replication were used. After the application, the larvaes placed Petri dishes which have perforated covers for the fresh air and kept in a climate room in a condition of 16:8 h (L/D) photoperiod, 26 ± 1 °C temperature, and 60-65% relative humidity for seventy-two hours. Fresh potato leafs provided as food in this period and after seventy-two hours dead-live counts were made. The experiments were repeated if 90% mortality ratio at the highest dose and 90% live ratio at the lowest dose were not provided. For thispurpose, 3, 3, 1, 1, 1, 1 populations were collected from Sandikli, Suhut, Dinar, Central, Bolvadin, Emirdag districts, respectively, which have the highest potato production areas in Afyonkarahisar province, and the populations were brought to Acarology Laboratory, Department of Plant Protection, Faculty of Agricultural Sciences and Technologies, Isparta University of Applied Sciences. The adults of the colorado potato beetle were collected from the field and cultured in cages in a climate room where 25 ±2 °C temperature, 60 ± 10, 16: 8 hours photoperiod conditions were provided. The 3rd instar larvaes used in experiments were obtained from these adults. A susceptible Coloradopotato beetle population obtained from Agriculture and Agri-food Canada (LondonDevelopment and Research Center) and cultured in our climate room was used as a reference population. The susceptible population has been rearing for more than 20 years and over 190 generations in climate rooms without any insecticide exposure. Resistancerates of populations were calculated by ratio the LD50 values of the field populations to the LD50 value of the susceptible population. Resistance rates determined for imidacloprid in Sandikli 1, Sandikli 2, Sandikli 3, Suhut 1, Suhut 2, Suhut 3, Dinar, Cental, Bolvadin, Emirdag populations as 3.96, 7.43, 11.33, 14.33, 27.31, 25.08, 9.12, 2.34, 7.46, 8.72-fold, respectively.