Determination of triacylglycerols in milk fat from different species using UPLC-Q-TOF-MS

Karrar E., Ahmed I. A. M., Huppertz T., ÖZ F., Wei W., Wang X.

INTERNATIONAL DAIRY JOURNAL, cilt.133, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 133
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.idairyj.2022.105405
  • Dergi Adı: INTERNATIONAL DAIRY JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
  • Atatürk Üniversitesi Adresli: Evet

Özet

Triacylglycerols (TAGs) are the major component of milk lipids and play important technological, nutritional and physiological roles. In this study, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used to characterise TAGs in cow, yak, donkey, goat, and camel milk fat. TAG separation based on partition number was achieved through an acetonitrile-isopropanol gradient elution. A total of 47, 52, 61, 75, and 87 TAGs were identified in camel, goat, donkey, cow, and yak milk fat, respectively, by UPLC-Q-TOF-MS. The most abundant TAG species in cow, yak, donkey, goat, and camel milk fat was 38:1, 40:3, 38:1, 40:1, and 52:2, respectively. TAG analysis using UPLC-Q-TOF-MS proved its potential to analyse different kinds of milk fat. This work contributes to a better understanding of the TAGs found in milk from various species.(c) 2022 Elsevier Ltd. All rights reserved.