Chitosan-poly (lactide-co-glycolide) (CS-PLGA) nanoparticles containing metformin HCl: Preparation and in vitro evaluation

Nuran G., Çetin M.

Pakistan Journal of Pharmaceutical Sciences, cilt.27, ss.1923-1929, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27
  • Basım Tarihi: 2014
  • Dergi Adı: Pakistan Journal of Pharmaceutical Sciences
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1923-1929
  • Anahtar Kelimeler: PLGA, CS, Nanoparticles, FT-JR, metformin HCl, PARTICLE-SIZE, MICROSPHERES, HYDROCHLORIDE, FORMULATION, MICROPARTICLES, CANCER
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, the preparation and in vitro characterisation of metformin HCl-loaded CS-PLGA nanoparticles (NPs) were aimed. The prepared nanoparticles (blank nanoparticles (C-1), 50 mg of metformin HCl loaded nanoparticles (C-2) and 75 mg of metformin HCl loaded nanoparticles (C-3) ranged in size from 506.67 +/- 13.61 to 516.33 +/- 16.85 rim and had surface charges of 22.57 +/- 1.21 to 32.37 +/- 0.57 mV. Low encapsulation efficiency was observed for both nanoparticle formulations due to the leakage of metformin HCl to the external medium during preparation of nanoparticles. Nanoparticle formulations showed highly reproducible drug,release profiles. similar to 20% of metformin HCl was released within 30 minutes and approximately 98% of the. loaded metformin HCl was released at 144 hours in a phosphate buffer (PB; pH 6.8). No statistically significant difference was noted between the in vitro release profiles of the nanoparticles (C-2 and C-3) containing metformin HCl. Also, nanoparticles were characterised using FT-IR and DSC.