Akademik Veri Yönetim Sistemi
VETERINARY RESEARCH COMMUNICATIONS, cilt.28, sa.6, ss.515-524, 2004 (SCI-Expanded)
This study examined the disposition kinetics and bioavailability of florfenicol after intravenous (i.v.), intramuscular (i.m.) and oral administration to rabbits at a dose of 30 mg/kg BW. Serial blood samples were collected through an indwelling catheter intermittently for 24 h for various routes. Plasma antibacterial concentrations were determined using a microbiological assay method with Bacillus subtilis ATCC 6633 as a reference organism. Plasma concentration-time data generated in the present study were analysed by non-compartmental methods based on statistical moment theory. Following i.v. administration, the overall elimination half-life (t(1/2beta)) was 1.54 h, mean residence time (MRT) was 1.69 h, mean volume of distribution at steady-state (V-dss) was 0.57 L/kg, and total body clearance (Cl-tot) was 0.34 L/kg/h. After i.m. and oral dosing, the terminal part of the curve should correspond to the absorption phase, instead of to the elimination phase, with terminal half-lives of 3.01 and 2.57 h, respectively The mean absorption time (MAT) was 2.65 h for i.m. and 2.01 h for oral administration. Elimination rate constants differed with i.v., i.m. and oral administrations, suggesting a flip-flop situation. The observed mean peak plasma concentrations (C-maxobs) were 21.65 and 15.14 mug/ml achieved at a post-injection time (T-maxobs) of 0.5 h following i.m. and oral dosing, respectively The absolute systemic availabilities were 88.25% and 50.79%, respectively, and the extent of plasma protein binding percent was 11.65%.