Measurement of antioxidant ability of melatonin and serotonin by the DMPD and CUPRAC methods as trolox equivalent

Gulcin I.

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, cilt.23, sa.6, ss.871-876, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 6
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1080/14756360701626223
  • Dergi Adı: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.871-876
  • Anahtar Kelimeler: Melatonin, serotonin, radical scavenging, DMPD, Cuprac method, IN-VITRO ANTIOXIDANT, ANTIRADICAL ACTIVITIES, CARBONIC-ANHYDRASE, VITAMIN-E, CAPACITY, DERIVATIVES, RECEPTORS, OXYGEN, ACID
  • Atatürk Üniversitesi Adresli: Evet

Özet

Melatonin (N-acetyl-5-methoxytryptamine) is the chief secretory product of the pineal gland and synthesized enzymatically from serotonin (5-hydroxytryptamine). These indoleamine derivatives play an important role in the prevention of oxidative damage. In the present study, DMPD radical scavenging and cupric ion (Cu2+) reducing ability of melatonin and serotonin as trolox equivalent antioxidant activity (TEAC) was investigated. Melatonin and serotonin demonstrated 73.5 and 127.4g/mL trolox equivalent DMPD+ scavenging activity at the concentration of 100g/mL. Also, at the same concentration, melatonin and serotonin showed 14.41 and 116.09g/mL trolox equivalent cupric ion (Cu2+) reducing ability. These results showed that melatonin and serotonin had marked DMPD+ radical scavenging and cupric ions (Cu2+) reducing ability. Especially, serotonin had higher DMPD radical scavenging and cupric ions (Cu2+) reducing activity than melatonin because of its phenolic group.