Identification of three strains of Mycobacterium species isolated from clinical samples using fatty acid methyl ester profiling

Özbek A., AKTAŞ O.

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, cilt.31, sa.2, ss.133-140, 2003 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 2
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1177/147323000303100210
  • Dergi Adı: JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.133-140
  • Anahtar Kelimeler: Mycobacterium, fatty acid profile, microbial identification system, GAS-LIQUID-CHROMATOGRAPHY, ALCOHOLS, DIFFERENTIATION, TUBERCULOSIS, XENOPI
  • Atatürk Üniversitesi Adresli: Evet

Özet

The cellular fatty acid profiles of 67 strains belonging to three different species of the genus Mycobacterium were determined by gas chromatography of the fatty acid methyl esters, using the MIDI Sherlock(R) Microbial Identification System (MIS). The species M. tuberculosis, M. xenopi and M. avium complex were clearly distinguishable and could be identified based on the presence and concentrations of 12 fatty acids: 14:0, 15:0, 16:1omega7c, 16:1omega6c, 16:0, 17:0, 18:2omega6,9c, 18:1omega9c, 18:0, 10Me-18:0 tuberculostearic acid, alcohol and cyclopropane. Fatty acid analysis showed that there is great homogeneity within and heterogeneity between Mycobacterium species. Thus the MIS is an accurate, efficient and relatively rapid method for the identification of mycobacteria.