Purification and Characterization of Laccase from newly isolated Thermophilic Brevibacillus sp (Z1) and its applications in removal of Textile Dyes

BOZOGLU C., ADIGÜZEL A., NADAROĞLU H., YANMIS D., GÜLLÜCE M.

RESEARCH JOURNAL OF BIOTECHNOLOGY, cilt.8, sa.9, ss.56-66, 2013 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 9
  • Basım Tarihi: 2013
  • Dergi Adı: RESEARCH JOURNAL OF BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.56-66
  • Anahtar Kelimeler: Laccase, Brevibacillus, Purification, Characterization, Removal textile dyes, EXTRACELLULAR LACCASE, FUNGAL LACCASES, PHENOL OXIDASE, AZO DYES, STREPTOMYCES, OXIDATION, SEQUENCE, DECOLORIZATION, GENOME, STRAIN
  • Atatürk Üniversitesi Adresli: Evet

Özet

Laccases are multicopper-containing oxidases (EC 1.10.3.2.) and they are widely found in fungi, higher plants and bacteria. Because of their high pH stability and thermostability, laccases from this strain have a potential for the treatment of textile bleaching effluents. In this research, the thermophilic test strain isolated from the Agri-Diyadin thermal springs, in the east of Turkey, were subjected to analysis of 16S rRNA sequence. The obtained sequence data were compared with data from the database in the Genebank and as a result it has been identified that this organism is a member of Brevibacillus genus (GenBank number KC292196). The laccase from Brevibacillus sp. (Z1) was purified by using precipitate of saturation (NH4)(2)SO4, DEAE-cellulose and Sephacryl S200. The purification degree and the molecular mass of the enzyme (93 and 110 kDa) were determined by SDS-PAGE and gel filtration chromatography.