Cytotoxic and antioxidant properties of essential oil of Centaurea behen L. in vitro

Celikezen F. C., Hayta S., Ozdemir O., Turkez H.

CYTOTECHNOLOGY, cilt.71, sa.1, ss.345-350, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 71 Sayı: 1
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1007/s10616-018-0290-9
  • Dergi Adı: CYTOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.345-350
  • Anahtar Kelimeler: Centaurea behen L, Asteraceae, Cytotoxicity, Oxidative effect, Peripheral human blood, SESQUITERPENE LACTONES, CHEMICAL-COMPOSITION, ANTIMICROBIAL ACTIVITY, SECONDARY METABOLITES, ASTERACEAE, TOXICITY, GENOTOXICITY, LYMPHOCYTES, CULTURES, CNICIN
  • Atatürk Üniversitesi Adresli: Hayır

Özet

Centaurea species of Asteraceae family are widely use in traditional medicine. Despite wide medicinal use of Centaurea sp., there is limited knowledge concerning Centaurea behen toxicity. Therefore, in this study, it is aimed to determine cytotoxic and oxidative effects of essential oil of C. behen on human blood cell cultures. 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays were performed to determine cytotoxic effects. In addition, total antioxidant capacity (TAC) and total oxidative status (TOS) were examined to determine oxidative potentials. The results indicated that all tested concentrations of essential oil of C. behen were cytotoxic and led to decreases of cell viability in both assays. Besides, C. behen led to significant increases of TOS levels and decreases of TAC levels. As a conclusion, the present study showed for the first time the cytotoxic and oxidant effects of essential oil of C. behen on cultured human whole blood cells.