Humic acid effects on retrotransposon polymorphisms caused by zinc and iron in the maize (Zea mays L.) genome

Yiğider E., Taşpınar M. S., Aydın M., Ağar G.

CEREAL RESEARCH COMMUNICATIONS, cilt.49, sa.2, ss.193-198, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: 2
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s42976-020-00111-3
  • Dergi Adı: CEREAL RESEARCH COMMUNICATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.193-198
  • Anahtar Kelimeler: Genomic template stability, Iron, Retrotransposon polymorphism, Zinc, Humic acid, INDUCED OXIDATIVE STRESS, HEAVY-METAL STRESS, DNA METHYLATION, FUNCTIONAL-ANALYSIS, PLANT-RESPONSES, PROTECTIVE ROLE, TOXICITY, CADMIUM, DAMAGE, GENOTOXICITY
  • Atatürk Üniversitesi Adresli: Evet

Özet

Long terminal repeat (LTR) retrotransposons are important pathways to examine various genome reorganizations based on environmental factors. The maize genome is one of the best representative examples. The aim of this study was to examine humic acid (HA) protective effects on genomic template stability (GTS) and LTR retrotransposon polymorphisms in corn seeds subjected to zinc (Zn) and iron (Fe) stress. In this study, maize seedlings were exposed to three doses (20, 40, and 60 mM) of ZnSO4 center dot 7H(2)O and FeSO4 and their combinations at 1500 ppm (0.075 g) HA. Inter-retrotransposon amplified polymorphism and retrotransposon-microsatellite amplified polymorphism techniques were used for genetic analyses. Results indicated that in all doses used, Zn and Fe increased retrotransposon polymorphisms and decreased the percentage of GTS via DNA damage. However, treatment of HA together with Zn and Fe resulted in decreased DNA damage and retrotransposon polymorphisms and increased GTS. These observations suggest that HA can be applied to reduce toxic effects in agricultural areas polluted with heavy metals.