Phytase from Weissella halotolerans: purification, partial characterisation and the effect of some metals

demir Y., Kotan M., DİKBAŞ N., Beydemir S.

INTERNATIONAL JOURNAL OF FOOD PROPERTIES, cilt.20, ss.2127-2137, 2017 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1080/10942912.2017.1368547
  • Dergi Adı: INTERNATIONAL JOURNAL OF FOOD PROPERTIES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2127-2137
  • Anahtar Kelimeler: Phytase, Enzyme purification, Characterisation, Weissella halotolerans, Metal ions, MICROBIAL PHYTASE, ACID, DIGESTIBILITY, EXPRESSION, STRAIN
  • Atatürk Üniversitesi Adresli: Evet

Özet

In the study, phytase was purified in three simple steps comprising ammonium sulphate precipitation, anion exchange and gel filtration chromatography from Weissella halotolerans. The enzyme was obtained with a specific activity of 227.73 EU/mg and 6.52% recovery. The molecular mass of the enzyme was determined to be 41.52 kDa. The optimum pH and temperature for the enzyme were 6.0 and 50 degrees C, respectively. Furthermore, the effects of metal ions on the enzyme were investigated. Ag+, Zn2+, Cr2+ and Fe2+ ions inhibited phytase by 21.86, 25.63, 32.82 and 90.43%, respectively, whereas Co2+, Cu2+, Pb2+, Cd2+ ve Mn2+ ions increased the enzyme activity.