Atıf İçin Kopyala
Uysal H., Çelik H., Kızılet H., Yılmaz B., Özdal M., Gülmez O.
Drosophila Information Service, cilt.103, sa.1, ss.1-9, 2020 (Hakemli Dergi)
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Yayın Türü:
Makale / Tam Makale
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Cilt numarası:
103
Sayı:
1
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Basım Tarihi:
2020
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Dergi Adı:
Drosophila Information Service
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Derginin Tarandığı İndeksler:
Other Indexes
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Sayfa Sayıları:
ss.1-9
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Atatürk Üniversitesi Adresli:
Evet
Özet
- Lactarius indigo is an edible fungus and contains various secondary metabolites. One of these metabolites, Guaiazulene (GUA), is a kind of terpene. In this study, it was observed that Drosophila melanogaster shortened the life span of both male and female populations based on Guaiazulene's dose-time interaction (25, 50, 100, 200 ppm GUA and chronic application every three days). The average life span in the control group was 48.83 ± 1.95 days in the ♀ population and 48.30 ± 2.22 days in the ♂ population. These values in the ♀♀ were found to be 44.91 ± 1.45 to 12.11 ± 0.26 in the lowest and highest Gua application groups (25-200 ppm); in ♂♂, it decreased from 44.70 ± 1.22 to 11.44 ± 0.21 (P < 0.05). In the second part of the study, water extracts of two edible fungi, Pleurotus sajor-caju (PSCwt) and Pleurotus osteratus (POwt), were used together with GUA (200 ppm GUA+PSCwt and GUA+POwt, 1:1 v/v ratio). Both extracts showed a healing effect and increased longevity compared to 200 ppm GUA application.
- Namely, in GUA+PSCwt application, the longevity was extended to 21.93 ± 0.62 days for females and 19.05 ± 0.46 days for males. These values were found as 21.83 ± 0.66 and 18.75 ± 0.39 days in GUA+POwt application, respectively (P < 0.05). In this study, both fungal extracts were also analyzed by gas chromatography-mass spectrometry (GS-MS). A total of 13 and 10 components were identified in POwt and PSCwt, respectively. In addition, total phenol and flavanoid contents of two extracts were determined.