Akademik Veri Yönetim Sistemi
Small Ruminant Research, cilt.230, 2024 (SCI-Expanded)
Pulmonary adenomatosis infections are an infectious and neoplastic disease that affects the respiratory system in sheep. Although the existence and prevalence of this disease have been revealed in studies, there is no definitive treatment today. Diagnosis of the disease; It can be detected by clinical, macroscopic, microscopic, and immunohistochemical methods. Although there are studies reporting the existence and prevalence of the disease today, the pathogenesis has not been fully elucidated. In this study, the effects of pulmonary adenomatosis (PA) infections on lung tissue oxidative DNA damage, inflammation, apoptosis, and autophagy were investigated in sheep. Care was taken to ensure that the lung samples used as negative controls did not show any macroscopic signs of pneumonia. The presence of tumoral foci was examined in the lung tissues to be used as positive control. Then the samples were examined histopathologically. All samples obtained were examined immunohistochemically for the presence of Jaagsiekte Sheep Retrovirus (JSRV) antibodies. In the study, the expression levels of IL1-β, Tnf-α, 8-OHdG, Bcl-2, BAX, Caspase3, LC3B, and LC3A were examined in 6 negative and 12 positive lung tissue samples for PA. In the lung tissues, proliferation of bronchiolar and alveolar epithelial cells, as well as alveolar macrophages and neutrophil leukocyte cell infiltration in their lumens, were observed. It revealed that severe OPA cases had more severe levels of IL1-β, Tnf-α, 8-OHdG, BAX, Caspase3, LC3A, and LC3B expressions than mild cases did. As a result of the research, in cases where the severity of tumor foci due to PA in lung tissues increased; Oxidative stress, inflammation, apoptosis, and autophagy were found to be significantly increased.