Effect of alpha-tocopherol oil antioxidant enzyme activities and lipid peroxidation in rainbow trout (Oncorhynchus mykiss)

Gulcin I., BEYDEMIR S., HISAR O.

ACTA VETERINARIA HUNGARICA, cilt.53, sa.4, ss.425-433, 2005 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53 Sayı: 4
  • Basım Tarihi: 2005
  • Dergi Adı: ACTA VETERINARIA HUNGARICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.425-433
  • Anahtar Kelimeler: alpha-tocopherol, antioxidant enzyme, lipid peroxidation, trout, VITAMIN-E, OXIDATIVE STRESS, IN-VITRO, PLASMA, L., FISH, OSTEICHTHYES, LIVER
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this Study, the effect of alpha-tocopherol on the antioxidant capacity of rainbow trout (Oncorhynchus mykiss) was determined. For this purpose, activities of antioxidant enzymes Such as catalase (CAT), peroxidase (POD) and glutathione reductase (GSSG-Rx) were investigated. Enzyme activities were measured at 0 (control), 1, 3 and 5 h after alpha-tocopherol injection. In addition, the levels of malondialdehyde (MDA) as a lipid peroxidation marker were determined in the erythrocytes. The results showed that alpha-tocopherol significantly activated the CAT, POD and GSSG-Rx enzymes as compared with the enzyme activities found in the controls (p < 0.05). However, MDA levels were significantly decreased by alpha-tocopherol treatment (p < 0.05). The results Suggest that alpha-tocopherol may have a pro-oxidant tendency at a high dose and cause mild oxidative stress which Could modulate signal transduction cascades, redirect gene expression, and influence Many Cellular responses such as proliferation, differentiation, and reproduction. For this reason, a-tocopherol should be used carefully in all applications in relation to fish.