Akademik Veri Yönetim Sistemi
Second International Congress on Biological and Health Sciences, Afyonkarahisar, Türkiye, 24 - 27 Şubat 2022, ss.32-41
The main source of industrial enzymes is microorganisms. The use of microorganisms in the production of enzymes has some important advantages. Compared to enzymes derived from herbal and animal sources, microbial enzymes can demonstrate higher stability under extreme conditions and can be produced in higher quantities. In addition, the production of microbial enzymes can be carried out at low cost using organic wastes.Thermophilic microorganisms are considered as a crucial source of lipase, especially of industrial importance. Lipases produced by thermophilic microorganisms exhibit a very stable structure and high activity at high temperatures in organic solvents. Moreover, these lipases show high resistance to chemical denaturation and can have high activity at alkaline pH values. Due to their high activity and stability at alkaline pH values and elevated temperatures, thermophilic lipases are mainly utilised in the detergent industry. Therefore, isolation, identification and characterization of thermophilic bacteria play an important role for the production of thermophilic lipase. In this study, lipase production and optimization was carried out with Anoxybacillus flavitermus MOB isolated from hot springs using cooking oil as a substrate. As a result of analysis, the optimal conditions were determined as; 3 g/L fish peptone, pH:7, 72 hours incubation time and temperature 50 oC. Under optimized conditions lipase enzyme activity was increased approximately 3-fold (316 U/mL).