EVALUATION OF BIOLOGICAL ACTIVITIES AND GC-MS ANALYSIS OF Zygophyllum eurypterum


SHAHZAD F., ZUBAIR M., RIZWAN K., RASSOL N., TAREEN R. B., RIAZ M., ...Daha Fazla

OXIDATION COMMUNICATIONS, cilt.38, ss.1978-1994, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38
  • Basım Tarihi: 2015
  • Dergi Adı: OXIDATION COMMUNICATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1978-1994
  • Atatürk Üniversitesi Adresli: Evet

Özet

For assessment of biological activities of extracts of stem and levaes of Zygophyllum eurypterum, a systematic procedure was adopted. Antioxidant activities were examined by evaluating DPPH radical scavenging activity, total phenolic contents (TPC), total flavonoids contents (TFC) and reducing power. The value of TPC observed was from 5.52 mg/100 g (n-hexane) to 98.25 mg/100 g (methanol) for leaves. Similarly the range of TFC was from 1.46 mg/100 g (n-hexane) to 24.41 mg/100 g (methanol) for stem and 0.70 mg/100 g (n-hexane) to 4.46 mg/100g (methanol) for leaves. The maximum values of IC50 were observed in n-hexane fraction, 80.41 for stem and 424.4 for leaves. Methanolic extracts show minimum values as 15.37 for stem and 84.61 for leaves. The % inhibition in linoleic acid system for stem was in the range of 26.7 (n-hexane) to 96 (methanol) and for leaves from 44.7 (n-hexane) to 87.5 (n-butanol). The % age inhibition in linoleic acid in leave and stem of Z. eurypterum was very close to standard BHT (92.8). Methanolic extract and residue show maximum value of antibacterial activity against Streptococcus (21 mm) and S. aureus (22 mm) for stem and n-hexane and residue against E. coli (21.1 and 20.4 mm). Similarly methanolic extracts (stem) show maximum value of antifungal activity against A. niger (19.2 mm) and n-hexane fractions show minimum values against C. albicus (9.8 mm). It was observed that methanolic extracts (stem) show minimum value of MIC against S. aureus (0.78 mg/ml) with maximum inhibition zone (22 mm). GC-MS analysis of essential oil of plant showed the presences of many compounds.