Determination of Developmental Stages of Cryptosporidium parvum in HCT-8 Cell Culture by Differential Interference Contrast Microscopy, Giemsa and Haematoxylin-Eosin Staining Methods

Kar S., Guven E., Yilmazer N., Karaer Z.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.17, sa.2, ss.171-175, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 2
  • Basım Tarihi: 2011
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.171-175
  • Anahtar Kelimeler: Cryptosporidium, Cell culture, Giemsa, Haematoxylin-Eosin, IN-VITRO, OOCYSTS, APOPTOSIS, CULTIVATION, APICOMPLEXA, INFECTION, LINE
  • Atatürk Üniversitesi Adresli: Hayır

Özet

This study aimed to compare the advantages of differential interference contrast microscopy, Giemsa and haematoxylin-eosin staining techniques for detecting, visualizing and distinguishing the developmental stages of Cryptosporidium parvum in cell culture. Data showed that interference contrast microscopy and Giemsa staining have certain advantages, whereas haematoxylin-eosin staining followed by formalin fixation clearly reveals certain changes occurred in the parasite. In the investigations, it was revealed that direct microscopy is effective in accurate definition of meronts and microgamets, but staining methods can differentiate meronts only.