Reticulated Ferrocene−Polycarbazole Nanofibers Coupled with CuAl-LDH for Ultrasensitive Label-Free Electrochemical Detection of CA125
ACS Applied Nano Materials, cilt.9, sa.40, ss.18477-18487, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 9 Sayı: 40
- Basım Tarihi: 2026
- Doi Numarası: 10.1021/acsanm.6c02757
- Dergi Adı: ACS Applied Nano Materials
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, Compendex
- Sayfa Sayıları: ss.18477-18487
- Anahtar Kelimeler: CA125 detection, electrochemical immunosensor, electrospun, ovarian cancer, polycarbazole
- Atatürk Üniversitesi Adresli: Evet
Özet
The development of highly sensitive and reliable sensing platforms for ovarian cancer biomarkers is of considerable importance for improving the early diagnostic performance and supporting clinical decision-making. Cancer antigen 125 (CA125) is among the most critical tumor markers for early detection and diagnosis. This study reports the synthesis and characterization of ferrocene-appended carbazole monomer and ferrocene-appended polycarbazole (PFC) using analytical techniques such as NMR, MALDI-MS, FTIR, single-crystal X-ray diffraction, UV−vis spectroscopy, TGA, and SEM. Carbazole was chosen for its superior conductivity, while ferrocene was integrated for its remarkable electroactivity. Subsequently, a blend of PFC and polycaprolactone (PCL) was electrospun onto a glassy carbon electrode (GCE), creating a reticulated PFC@PCL/GCE surface with enhanced electrochemical performance. CuAl-layered double hydroxide (CuAl-LDH) was synthesized and functionalized with amine (−NH2) groups (CuAl@A) via functional organosilane, creating a highly effective immobilization matrix for anti-CA125. The structural and morphological properties of CuAl-LDH and CuAl@A were characterized with SEM, TEM, FTIR, XRD, and TGA. CuAl@A was drop-cast onto the reticulated electrode surface to create a suitable platform for immobilizing anti-CA125, resulting in a modified immunosensor (Ab-CA125/CuAl@A/PFC/GCE). Under the optimized conditions, the immunosensor exhibited high selectivity and sensitivity, enabling the detection of CA125 at low concentrations. The sensor demonstrated a broad linear detection range from 1.0 pg/mL to 100 ng/mL with a detection limit of 0.62 pg/mL. The validation of the proposed electrochemical immunosensor system was performed with spike tests and a reference method (ELISA), both of which demonstrated high accuracy. This label-free electrochemical immunosensor represents a promising platform for the clinical detection of tumor markers, offering a simple, reliable, and highly sensitive approach for CA125 quantification.