Akademik Veri Yönetim Sistemi
Gümüşhane Üniversitesi Fen Bilimleri Dergisi, cilt.12, sa.4, ss.1093-1101, 2022 (Hakemli Dergi)
The rising application of silver (Ag) nanoparticles in many sectors such as food, medicine, and agriculture lead to toxic effects on the ecological environment. Thus, studies on biological synthesis methods are carried out in order to diminish the toxicity caused by Ag nanoparticle synthesis methods. However, studies on the toxicity of biosynthesized Ag nanoparticles on fish cell lines are very few. The purpose of this research was to perform the biological synthesis of Ag nanoparticles via Eruca vesicaria plant extract and to examine their toxicity in rainbow trout gonad cell line-2 (RTG 2). The characterization of Ag nanoparticles obtained from E. vesicaria was done by UV-vis, TEM, and XRD. The toxicity of Ag nanoparticles (100 µg/mL- 6,25 µg/mL) in the RTG-2 cell for 24 hours was determined by sulforhodamine B assay. Ag nanoparticles obtained in the form of a sphere, triangle, cube, and sizes of 5-20 nm showed significant a toxic effect on RTG-2 fish cells depending on the dose at p≤0,001 levels. This study is important in terms of proving that Ag nanoparticles obtained by biological synthesis have a toxic effect on fish cell lines and showing that there is a need for studies to reduce the release of Ag nanoparticles to the environment rather than synthesis methods.