ICP-MS and HPLC analyses, enzyme inhibition and antioxidant potential of Achillea schischkinii Sosn.

Türkan F., Atalar M. N., Aras A., GÜLÇİN İ., Bursal E.

Bioorganic Chemistry, cilt.94, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 94
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.bioorg.2019.103333
  • Dergi Adı: Bioorganic Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chimica, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Achillea schischkinii, Antioxidant, Elemental analysis, Enzymes, Phenolic compounds, GLUTATHIONE-S-TRANSFERASE, IN-VITRO ANTIOXIDANT, PHENOLIC-COMPOUNDS, HEAVY-METALS, ACETYLCHOLINESTERASE, BUTYRYLCHOLINESTERASE, IDENTIFICATION, CAPACITY, LEAVES, PLANTS
  • Atatürk Üniversitesi Adresli: Evet

Özet

Achillea schischkinii Sosn. is an endemic plant species and it belongs to Asteraceae family. It is distributed widely in the Central and East Anatolia. This study was carried out for evaluation of the antioxidant activity, enzyme inhibition effect, elemental and phenolic content of A. schischkinii. Briefly, acetylcholinesterase (AChE), butyr-ylcholinesterase (BChE), alpha-glycosidase (alpha-Gly), and glutathione S-transferase (GST) enzymes were strongly inhibited by A. schischkinii. IC50 values for AChE, BChE, a-Gly, and GST enzymes were found as 19.3 mg/mL, 15.4 mg/mL, 69.3 mg/mL, and 34.7 mg/mL respectively. The antioxidant activity of the sample was evaluated by four different in vitro bioanalytical methods. Besides, the concentrations of twelve elements in A. schischkinii were analyzed by ICP-MS technique. Zn (50.6 ppm), Mn (23.0 ppm), and Cu (12.7 ppm) were found as major elements. Furthermore, catechin (20.8 mu g/mg extract), trans-ferulic acid (18.3 mu g/mg extract), and gallic acid (11.2 mu g/mg extract) were characterized as major phenolic compounds by using HPLC.