Simple high-performance liquid chromatography method for formaldehyde determination in human tissue through derivatization with 2,4-dinitrophenylhydrazine

YILMAZ B., AŞCI A., Kucukoglu K., ALBAYRAK M.

JOURNAL OF SEPARATION SCIENCE, cilt.39, sa.15, ss.2963-2969, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 15
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1002/jssc.201600345
  • Dergi Adı: JOURNAL OF SEPARATION SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2963-2969
  • Anahtar Kelimeler: Derivatization, Formaldehyde, Liquid-liquid extraction, Liver tissue, SOLID-PHASE MICROEXTRACTION, HPLC METHOD, FLUORESCENCE DETECTION, HUMAN PLASMA, GC-MS, FISH
  • Atatürk Üniversitesi Adresli: Evet

Özet

A simple high-performance liquid chromatography method has been developed for the determination of formaldehyde in human tissue. FA Formaldehyde was derivatized with 2,4-dinitrophenylhydrazine. It was extracted from human tissue with ethyl acetate by liquid-liquid extraction and analyzed by high-performance liquid chromatography. The calibration curve was linear in the concentration range of 5.0-200 mu g/mL. Intra-and interday precision values for formaldehyde in tissue were <6.9%, and accuracy (relative error) was better than 6.5%. The extraction recoveries of formaldehyde from human tissue were between 88 and 98%. The limits of detection and quantification of formaldehyde were 1.5 and 5.0 mu g/mL, respectively. Also, this assay was applied to liver samples taken from a biopsy material.