IN VITRO CYTOTOXICITY TEST METHODS: MTT and NRU

Şahin Mazlumoğlu B.

PharmATA, cilt.3, sa.2, ss.50-53, 2023 (Hakemli Dergi)

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 3 Sayı: 2
  • Basım Tarihi: 2023
  • Doi Numarası: 10.5152/pharmata.2023.1287964
  • Dergi Adı: PharmATA
  • Sayfa Sayıları: ss.50-53
  • Atatürk Üniversitesi Adresli: Evet

Özet

The use of in vitro cytotoxicity tests is increasing day by day with the increase in human exposure to chemicals because these tests involve less cost and less time than in vivo methods. The purpose of in vitro cytotoxicity tests is to detect cell viability. The toxicity of the xenobiotic applied to the cell should be determined. In vitro cytotoxicity tests are frequently used in analyses to detect cell viability, such as drug development and cancer research. This review focuses on MTT and Neutral Red Uptake analyses, which are the most commonly used in vitro cytotoxicity test methods. At the same time, how in vitro cytotoxicity tests are performed, where they are used, and what are the advantages and disadvantages of these tests are also reviewed in this article. The purpose of this article is to help researchers who will use the information about these frequently used tests by compiling them.