Serological Screening of Patients Diagnosed with Alveolar Echinococcus Disease in Their Home Regions

AKKAŞ Ö., USLU H., Yilmaz I., AYDIN Y., KORKUT E., YILMAZ A.

Iranian Journal of Parasitology, cilt.17, sa.4, ss.554-561, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 4
  • Basım Tarihi: 2022
  • Dergi Adı: Iranian Journal of Parasitology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.554-561
  • Anahtar Kelimeler: Echinococcosis, Echinococcus multilocularis, Western blot, Echinococcus alveolaris, IMMUNOSORBENT-ASSAY ELISA, AFFINITY-PURIFIED EM18, RECOMBINANT EM18, SERODIAGNOSIS, ANTIGEN, DIFFERENTIATION, MULTILOCULARIS, GRANULOSUS
  • Atatürk Üniversitesi Adresli: Evet

Özet

© 2022 Akkas et al. Published by Tehran University of Medical Sciences.Background: We aimed to determine the prevalence of Alveolar echinococcusis using ELISA in our region, to perform the specific differentiation of species by using the Western Blott method, to diagnose and treat this disease effectively in early periods, and to inform the patients about the results quickly. Methods: From the provinces of Erzurum, Kars, Ardahan, Iğdır, Ağrı and Erzincan in the Eastern Anatolia Region, Turkey in August-September 2017, blood samples were provided from 305 participants (volunteer patients and their relatives), including 151 females (49.50%), whose ages ranged between 6-85 yr and were diagnosed as Alveolar echinococcosis (AE) and operated in concerned clinics. Results: EIgG ELISA was determined as positive in 29 (9.5%) participants, including 17 (11.3%) females and 12 (7.8%) males. In addition, Em2-Em18 ELISA was also determined as positive in 15 (4.9%) participants, including 9 (6%) females and 6 (3.9%) males. Tweleve (3.94 %) of these findings were observed as positive in terms of both tests. Through the verification done by Anti-EWB IgG, Echinococcus multilocularis (EM) was identified in 7 (21.9%) of the female participants, E. granulosus (EG) in 1 (3.1%), and both parasites in 2 (6.2%). For male participants, EM was determined in 3 (9.3%) of them, EG in 1 (3.1%), and both parasites in 1 (3.1%). Conclusion: AE continues to threaten public health in the region. In families where AE is detected positive, the reason for disease is based on nutrition; thus, it will be proper to check up all family members in the terms of infection.