Determination of atenolol in human urine by using HPLC
SEPARATION SCIENCE PLUS, cilt.1, sa.1, ss.4-10, 2018 (ESCI)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 1 Sayı: 1
- Basım Tarihi: 2018
- Doi Numarası: 10.1002/sscp.201700023
- Dergi Adı: SEPARATION SCIENCE PLUS
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI)
- Sayfa Sayıları: ss.4-10
- Anahtar Kelimeler: atenolol, beta-blockers, liquid-liquid extraction, urine, validation, PERFORMANCE LIQUID-CHROMATOGRAPHY, BETA-BLOCKERS, HUMAN PLASMA, FLUORESCENCE
- Atatürk Üniversitesi Adresli: Evet
Özet
A simple high-performance liquid chromatography method has been developed for determination of atenolol in human urine. The method was validated on Ace C-18 column using fluorescence detection. The mobile phase consisted of methanol/water (50:50, v/v) containing 0.1% trifluoroacetic acid. Fluorescence detection was performed at 276 nm (excitation) and 296 nm (emission). The calibration curve was linear between the concentration range of 5-150 ng/mL. Intra- and inter-day precision values for atenolol in human urine were less than 6.09, and accuracy (relative error) was better than 5.50%. The extraction recoveries of atenolol from human urine were between 93.0 and 98.8%. The limits of detection and quantification of atenolol were 1.5 and 5 ng/mL, respectively. Also, the method was successfully applied to a patient with hypertension who had been given an oral tablet of 50 mg atenolol.