The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase

BEYDEMIR S., KULACOGLU D., CIFTCI M., Kufrevioglu O.

TURKISH JOURNAL OF CHEMISTRY, cilt.27, sa.5, ss.601-608, 2003 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 5
  • Basım Tarihi: 2003
  • Dergi Adı: TURKISH JOURNAL OF CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.601-608
  • Anahtar Kelimeler: sheep eye lens, dexamethasone, glucose-6-phosphate dehydrogenase, GLUCOCORTICOID-INDUCED CATARACT, HUMAN ERYTHROCYTES, SUPEROXIDE-DISMUTASE, ENZYME-ACTIVITIES, DEFICIENCY, PURIFICATION, INFLAMMATION, GLUTATHIONE, SARDINIA, PROTEIN
  • Atatürk Üniversitesi Adresli: Evet

Özet

Glucose-6-phosphate dehydrogenase (G6PD) enzyme was purified from sheep lenses, and the in vitro effects of dexamethasone on the enzyme's activity were investigated. Sheep lens glucose-6-phosphate dehydrogenase was purified 10,000-fold by using ammonium sulphate fractionation and 2',5'-ADP-Sepharose 4B affinity gel chromatography for a yield of 83.8% and a specific activity of 7.6 EU/mg protein. Enzyme activity was determined by Beutler's method. Dexamethasone strongly inhibited the enzyme under in vitro conditions. The I-50 value of the dexamethasone was 3.05 mM.