The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase


BEYDEMIR S., KULACOGLU D., CIFTCI M., Kufrevioglu O.

TURKISH JOURNAL OF CHEMISTRY, vol.27, no.5, pp.601-608, 2003 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 5
  • Publication Date: 2003
  • Journal Name: TURKISH JOURNAL OF CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.601-608
  • Keywords: sheep eye lens, dexamethasone, glucose-6-phosphate dehydrogenase, GLUCOCORTICOID-INDUCED CATARACT, HUMAN ERYTHROCYTES, SUPEROXIDE-DISMUTASE, ENZYME-ACTIVITIES, DEFICIENCY, PURIFICATION, INFLAMMATION, GLUTATHIONE, SARDINIA, PROTEIN
  • Ataturk University Affiliated: Yes

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) enzyme was purified from sheep lenses, and the in vitro effects of dexamethasone on the enzyme's activity were investigated. Sheep lens glucose-6-phosphate dehydrogenase was purified 10,000-fold by using ammonium sulphate fractionation and 2',5'-ADP-Sepharose 4B affinity gel chromatography for a yield of 83.8% and a specific activity of 7.6 EU/mg protein. Enzyme activity was determined by Beutler's method. Dexamethasone strongly inhibited the enzyme under in vitro conditions. The I-50 value of the dexamethasone was 3.05 mM.